multi-function dna purification kit bioteke cat Search Results


recovery kit  (Bioteke Corporation)
86
Bioteke Corporation recovery kit
Recovery Kit, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human tgf-β3, nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct  (SwitchGear Genomics)
90
SwitchGear Genomics human tgf-β3, nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
Human Tgf β3, Nppa, Negative Control (Scrambled), Or Positive Control (Actin) Luciferase Reporter Construct, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human tgf-β3, nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct/product/SwitchGear Genomics
Average 90 stars, based on 1 article reviews
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desktop microcentrifuges  (Eppendorf AG)
95
Eppendorf AG desktop microcentrifuges
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
Desktop Microcentrifuges, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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desktop microcentrifuges - by Bioz Stars, 2026-06
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115v benchmark scientific bv1000  (AutoMate Scientific Inc)
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AutoMate Scientific Inc 115v benchmark scientific bv1000
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
115v Benchmark Scientific Bv1000, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/115v benchmark scientific bv1000/product/AutoMate Scientific Inc
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115v benchmark scientific bv1000 - by Bioz Stars, 2026-06
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needle bd 305145 cotton  (AutoMate Scientific Inc)
86
AutoMate Scientific Inc needle bd 305145 cotton
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
Needle Bd 305145 Cotton, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/needle bd 305145 cotton/product/AutoMate Scientific Inc
Average 86 stars, based on 1 article reviews
needle bd 305145 cotton - by Bioz Stars, 2026-06
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Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and nppa (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. I: luciferase reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: The chromatin-binding protein Smyd1 restricts adult mammalian heart growth

doi: 10.1152/ajpheart.00235.2016

Figure Lengend Snippet: Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and nppa (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. I: luciferase reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].

Article Snippet: After 24 h, the cells were transfected with 75 ng of human TGF-β3, Nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct (SwitchGear Genomics) using FuGene HD (SwitchGear Genomics) at a 6:1 ratio to DNA and incubated for 48 h. Luciferase activity was assayed using the LightSwitch Luciferase assay reagent (SwitchGear Genomics) according to the manufacturer's instructions and measured using a BioTek Synergy Neo HTS Multi-Mode microplate reader.

Techniques: Functional Assay, Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction, Chromatin Immunoprecipitation, Luciferase, Reporter Assay, Negative Control